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If I have do.imp = TRUE enabled and 27% of a participant's time is classified as non-wear, but the day still meets the requirement of having >16 hours of valid data, is the entire non-wear period imputed with the average of the other days of that week?

Yes, they are imputed based on time of the day. The default of 16 hours is fairly low, but chosen in the context of studies where participants are asked to wear the accelerometer 24/7. Ideally the 16 hour criteria is only relevant to catch the days with really poor compliance, while for most days the participant still wears it for much more than 16 hours. So, if you see that a person consistently does not wear the accelerometer for a subs…

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@Malontax
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@vincentvanhees
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