v2.3.5

bugfix spot extraction

v2.3.2 minor fix

This release includes no pipeline/quantification modification.

Bugfix :

• Deleting all spots from a cluster no longer causes a recursion error.
• Deleting several clusters in a row by removing single molecules updates features table correctly.

v2.3.1 Co-localization fix

• Fixed bugs for co-localization from spot_extractions, added co-localization feature.
• Fixed bugs for batch mode.
• Fixed bugs for spot extraction
• Fixed bugs for segmentation using external nucleus image
• Fixed bugs for parameters inputs in detection and segmentation

v2.2.0 Segmentation logic update

During segmentation user can see other channels if this information is needed to better segmentation or discard cells.

Also if segmentation is modified during clustring interactive step new segmentation is used for quantification (as previously) and also is stored in memory to be saved or used for other detections.

Small Fish 2.1.14 - Stable release

v2.1.14

v2.1.14

Small Fish 2.1.9 - Stable release

Version tested on Linux (ubuntu), MacOS and windows..

Small Fish 2.1.0 - Python 3.12

• Software moved to python 3.12
• Comfort updates including custom preset, new interactive tools for detection and clustering.
• (beta-test) Autofluorescent background removing tool
• New segmentation options

Version 2.0 - 3D segmentation

v2.0.2

Delete .github/workflows/python-publish.yml

1.10.3 Stable

Version 1.10.3 including transcription site quantification tool and memory usage update for batch mode.

v1.9.3 - Zenodo archive

Publication on zenodo.org for scientific usage in publications.