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##Localized Reconstruction

Localized Reconstruction is a general method for the localized three-dimensional reconstruction of substructures bound to a larger particle. After determining the particle orientations via conventional methods, local areas corresponding to the subunits ('subparticles') can be extracted and treated as single particles.

###Getting started

• How to install

• How to run

• Frequently asked questions

###Using the script

The main input to the script is a STAR file from Relion 3D classification or 3D refinement. Normally the script is run in four steps: 1. Prepare particles. 2. Create subparticles. 3. Extract subparticles. 4. Reconstruct subparticles.

After these steps have been executed, the subparticle STAR file and 3D reconstruction can be used as inputs for 3D classification or 3D refinement of subparticles in Relion.