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Eugene Katrukha edited this page Jun 10, 2026 · 3 revisions

The plugin can build a kymograph along the bending/undulating
curves/lines/filaments (think microtubules, cilia, neurites, etc).

For example, here is a growing and bending microtubule
with kinesins running along it.

example movie

You can provide a reference ROIs at different time points of the movie,
shown here (6 ROIs for 100 frames).
example movie with reference ROIs

The plugin will morph (or interpolate) ROI shapes at all intermediate frames:
example movie with interpolated ROIs

And create a kymograph along them:
kymograph

Optionally, it will output a straightened "kymograph stack":
kymograph stack output

Developed in Cell Biology group of Utrecht University.
Check out Updates history. The plugin and this wiki are under constant development.
E-mail for any questions, feedback, errors or suggestion or tag @ekatrukha at image.sc forum.

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