Skip to content

Multiome_v6.0.0

Choose a tag to compare

View all tags
@github-actions github-actions released this 14 Apr 17:55
Multiome_v6.0.0
5283cc7
This commit was created on GitHub.com and signed with GitHub’s verified signature.
GPG key ID: B5690EEEBB952194
Verified
Learn about vigilant mode.

6.0.0

2025-04-02 (Date of Last Commit)

  • Implemented a unified STARsolo execution strategy to ensure consistent and accurate cell barcode correction across the entire dataset. This update resolves discrepancies that previously arose from sharded (partitioned) processing, where each shard independently corrected barcodes using incomplete local priors. By consolidating barcode frequency calculations and applying correction globally, the pipeline now mirrors the behavior of DropSeq and Cell Ranger pipelines
  • Refactored the STAR alignment step (STARsoloFastq) in Optimus and removed tasks FastqProcessing and MergeSortBamFiles
  • Removed MergeStarOutput tasks from Optimus pipeline; added necessary parts of MergeStarOutput task to the STAR alignment step (STARsoloFastq). Additional outputs added to STARsoloFastq task as a result; this includes row_index, col_index, sparse_counts, library_metrics, mtx_files, filtered_mtx_files and cell_reads_out
  • Updated the STAR docker image to include Samtools and Python
Assets 5
Loading