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merge feature addition to add number of ambiguous calculation and additional trace line on final plot by @lskatz. (pull request #1 )
fix bug: unique region parsing from bed/bedpe file. It was missing the first and last region and error when there is fully covered amplicon by another amplicon.
expose the min coverage as parameter to let user define the ambigous N and default to 10
fix bug: the interatcive plot with new added trace to show number of ambiguous.
