The `slot` argument of `Assays()` was deprecated in SeuratObject 5.0.0 and is now defunct.

[nbutyrate]

Hi, I am trying to analyze a Seurat object in the SCP package, and its throwing the following error.

Could you please take a look and let me know if its something you could help me resolve,

DIS_sub <- SCP::RunDEtest(srt = DIA_sub1, group_by = "Cell_Type", fc.threshold = 1, only.pos = FALSE)
[2023-08-20 18:48:35] Start DEtest
Workers: 14
Error:
! The slot argument of Assays() was deprecated in SeuratObject 5.0.0 and is now defunct.
ℹ Please use LayerData() instead.
Run rlang::last_trace() to see where the error occurred.

[longmanz]

Hi,
In Seurat V5, "slot" no longer exists. If you want to keep using SCP package, you may need to revert back to Seurat V4. Or you may need to wait for SCP's update so that it is compatible with Seurat V5.