Meeting 9 4 2020

Our next deadline is 9,10 Nov. Oversight meeting

Near-term Plan

0. Obtaining data

• Sexed matched pairs of complementary function. Priority is one sex matched pair.

1.a.

Pairings set #1 is Geneoglosis; Masseter; Phenic
Geneoglosis DK52 (F) - Done by Ed as of 26 Aug - Ch 1-3 HiRes ; need to verify overlays (Beth to make mtg w/Ed on 27 Aug - priority 1)
DK39 (M) - under Ed's resorting - soon to have Ch 2/3 HiRes; (Beth to make mtg w/Ed on 27 Aug)
Masseter DK54 (F) - need to correct for 6 µm sections; Beth and Hannah and David need to reasign sections; need to check Ch 2/3 HIRes - priority 2
DK43 (M) - need to correct for "squished" side + add Ch 2/3 HIRes (Beth to make mtg w/Ed on 27 Aug)

1.b.

Pairings set #2 is Vibrissa Intrinsic; Vibrissa (mystacial) Extrinsic.
Intrinsic DK51 (F)
Set #2 is NOT for the current meeting

2. Align section-to-section (local is Elastix on 32X rediced sectios extrapolated to full section - so long as sectios are OK) We are still working out the pipeline with CSHL/Bangalor.
3. In short term - align atlas manually ; DK and BF to do this for DK52 AFTER ED and BF verify sections and labeling
4. Display overlay data (Ch 2 and 3) using Neuroglancer (27 Uag Mtg to clarify this)
5. Access, display, and annotate DOTS! (labeled somata for now) - using CVAT
6. Assign each puncta an (x,y,z), and display in real units as a ball (same formal as with landmarks) in Neurglancer

Other tasks

1. Establish offsets for annotations to the three reference (Thionin) brains
2. Simultaneous share and evaluate data across sites with Neuroglancer
3. Add the shell of the brain