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To be able to run the pipeline you will need to install a range of R packages from CRAN, bioconductor and github. In addition you will need to have a few other packages installed on your server such as R4.1 or higher, git, gpg, sshpass, pinentr. If you have a module load system installed on your server you can check whether it has already been installed with module avail. If it is in the list of available software then you can load the package by for example: module load tools/git/2.35.1.
You can find more details here: System requirements.
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- System requirements and pipeline installation
0. Set up folders
1a. Check data
1b. Process genetic data
1c. Check methylation and phenotypes
1d. mQTL control GWAS
1e. GWAS of genetic PCs
1f. Cross-cohort normalisation of methylation data
1g. Compute haplotype components
1 upload. Upload to Bristol SFTP
2a. Relatedness assessment/removal
2b. Regular and variance EWAS
3a. Run meQTL analysis
3b. Run variance meQTL analysis