Fecal Microbiota Transplants (FMT)
Short link to this page: http://HumanMicrobiome.info/FMT
HumanMicrobes.org is trying to find the fewer than 0.1% of people who qualify to be a stool donor.
You can use the sidebar flair here to browse FMT studies, and more: https://old.reddit.com/r/humanmicrobiome/search?q=flair%3A%27FMT%27&sort=new&restrict_sr=on
Table of Contents:
- Scientific info
- General FMT articles & videos
- FMT Clinics
Short link to this section: HumanMicrobiome.info/FMT#Screening
International consensus conference on stool banking for faecal microbiota transplantation in clinical practice (Nov 2019) https://gut.bmj.com/node/226914.full#boxed-text-2
European consensus guidelines tests (Mar 2017): http://gut.bmj.com/content/66/4/569#boxed-text-3
OpenBiome donor screening/testing info: http://www.openbiome.org/s/The-OpenBiome-Quality-Safety-Program.pdf - http://www.openbiome.org/s/Quality-Metrics.pdf
Do not solely rely on testing (conventional or 16s) as it is very limited in value: http://HumanMicrobiome.info#Testing
If you cannot find a participating doctor, the tests can be ordered directly (have to pay cash yourself though, probably a few hundred dollars) from http://requestatest.com/ or https://www.ultalabtests.com/Patient. In the UK you can use https://www.medichecks.com.
For the stool testing portion you can use https://www.mymedlab.com or https://mylabsforlife.com to get the Diagnostic Solutions GI Map test. In the EU you can try https://ifu-wolfhagen.de/ or https://smartnutrition.co.uk/ to get the GI Map.
If you look at their Sample Report, it has all of the required stool tests and most of the "specific situations" stool tests listed in the EU guidelines. The donor will need to create an account on mymedlab and share the login info with you so you can order/pay for the test. If you get this test through a provider you need to specifically ask them to check off on the order the "beta-lactamase resistance" section, or "Antibiotic Resistance Genes, genotypes". Mymedlab said "when you receive your kit, you can make the change on the requisition form".
Blood testing can typically be done at a free STD clinic. https://gettested.cdc.gov/
Thepowerofpoop.com recommends Direct Labs - the "Labs for Fecal Diversity Tests Only" aren't that useful in my opinion (see the "testing" section of this wiki). The "Comprehensive Digestive Stool Analysis 2.0™ (CDSA 2.0)-Genova Kit" is probably the most useful directlabs test.
A few things to keep in mind when looking for a donor:
- The gut microbiome is heritable and gets passed down generationally. Including dysbiosis, pathogens, and extinctions from poor diet and antimicrobials. A child with no lifetime antimicrobial use may not actually have an eubiotic, disease resistant gut microbiome, but rather they may have just not yet been exposed to an infectious pathogen that necessitated an antimicrobial.
- There are differences between everything - sex, race, ethnicity, lifestyle, location, etc.. There is no evidence those differences (other than health-related ones, such as healthy diet and healthy lifestyle) matter for safety nor efficacy. Current evidence supports universal donors. Example for sex.
- Relatives can often have "pre disease" states of dysbiosis . Meaning they have dysbiosis themselves but not yet enough to trigger the disease of the family member that needs the transplant. So contrary to common misinformation, relatives are often not ideal. The EU consensus concluded universal donors are as good as any. And that is supported by this recent IBS clinical trial and this Mar 2020 systematic review.
- Both genetic and gut microbiome diversity are beneficial.
- There is a large amount of evidence (in this wiki) that donor quality (rather than compatibility) is the #1 factor. But of course each donor (and recipient) is very unique and thus some donors would have certain microbes needed to treat certain conditions/individuals, while others do not. And naturally there would be varying results when transplanting one unique microbiome into another. I compared it here to a jigsaw puzzle.
- Nearly anything wrong with a donor can be transferred via FMT.
- Donors often misjudge their stool quality. So you probably want to get a daily sample for a few days in a row before using any of it, just to judge the quality and consistency yourself. If a person is having unstable stools it's probably not safe.
- You absolutely cannot blindly trust donor quality from official sources such as stool banks, clinical trials, hospitals, etc. . You must learn what it means to be a high quality donor and verify that the provided donor is high quality. If they will not verify that information with you, do not use them.
Soft stools seem to be from one or more of:
- A low quality gut microbiome that lacks certain microbes needed in the digestion of certain foods. Or lacks certain microbes that control/limit others.
- A pathogen that feeds off certain food items (iron for example), and thus causes soft stools & other problems when eating those foods.
- Temporary stomach upset from either mild food poisoning or poor hygiene/sanitary practices.
I had no idea how important stool type & consistency was coming into this, but it seems high quality donors have identical stools, while low quality donor's stools are heterogeneous, thus it's one of the major ways to judge stool quality. This Anna Karenina hypothesis supports this. As do the majority of case reports I've seen, and this study .
This 2017 study saying otherwise is only for c.diff (which requires much less strict donor criteria). And it's quite likely (looking at the donor criteria) that studies like that lacked high quality donors, so they may have only been comparing average/low quality donors with each other. Also I believe it is a matter of "the highest quality donors all have type 3 stools, but not every type 3 stool donor is high quality".
Currently I believe it's likely that deficiencies in bile acid metabolism (missing microbes) is a primary cause of stool types other than dark type 3.
The 4 basic steps to donor screening (in this order):
- A detailed questionnaire.
- Verification of stool type and physical fitness. Stool type should be verified over at least 3 consecutive days.
- If it's not already someone you know well you should do a video or in-person interview. Try to get to know them a bit.
- Stool & blood testing.
The discussion section of this 2014 review paper "Rural and urban microbiota - To be or not to be?" provides excellent info on gut microbiome, FMT, and donor selection: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153773/
More info on what donor quality means, and why it's so hard to find high quality donors: https://archive.fo/I3wSb#selection-1883.59-1883.60
See the "impact factors" section under "scientific info" heading for more on donors, including supporting evidence for athletes.
Short link to this section: HumanMicrobiome.info/FMT#Procedure
Check out the video on this page for a good overview of ASU's recent FMT study, which was one of the best I've seen: https://autism.asu.edu/ - they used both oral and rectal routes.
The most common/major flaws with FMT studies/procedures are:
- Poor donor quality . Donors should be under 30 , have very limited life time antibiotic exposure (ideally none) , athletic & low body fat, and good mental health. Firm stool consistency seems important too. Typical donor criteria you see is a complete joke. Stuff like "no abx in past 3 months, 18-50 yrs old, no pathogens in stool & blood test".
- Insufficient treatment length. Many studies only do a single infusion, but for many people/conditions you might need to do it daily for 2+ months (Eg: ASU autism study).
- Too much oxygen exposure. Blending is quite common and this oxygenates the stool sample.
- Colon-only procedures. The small intestine is very important , so completely ignoring it is a likely flaw for some conditions .
Keep in mind that c. diff is a lot easier to cure than other conditions. So even though a study shows 'x' is effective for c.diff, other harder to cure conditions will likely need stricter criteria.
It's common for the recipient to feel feverish & chills, and possibly some initial diarrhea & other temporary side effects after the first 1-2 FMTs. It seems to be a sign of a high quality donor. Symptoms of a "changing of the guard".
The less delay between the donor having the BM and the recipient using it, the better. 15 minutes or less might be ideal, but it can be hard to always be there when your donor has a BM, so it's possible to use an insulated lunch bag like this:
Freezing the stool for either shipping or storage is common. Glycerol/Glycerin is the commonly used antifreeze additive, but it's also a laxative, and therefore problematic for some people. Maltodextrin is a good alternative. It can be bought in bulk online for around $4/lb. The ratios used in that study were approximately 2 tablespoons maltodextrin to 1/2 cup saline. Which can be mixed in with 2 tablespoons of stool at about a 4:1 ratio: 4 parts MD+saline, 1 part stool.
I found that 1/2 cup saline was way too much liquid, and 1tbls of saline to 2tbls maltodextrin seems to work well, and ends up pretty close to the consistency of pure glycerol. Premix the saline & maltodextrin, then mix it in with 2tbls of stool.
Alternatively (but probably less ideal), you could mix in a little bit of saline (close to 1/2 tbls saline per 1 tbls stool), little by little, mixing them in the ziplock bag the stool was collected in, to create a thick yogurt/custard-like consistency, then add maltodextrin till it's a doughy/firm consistency, which can then be encapsulated or frozen straight.
UPDATE: Here's a video demonstration https://www.youtube.com/watch?v=89FCAsqC8BY for making capsules, and mixing in the powder antifreeze. I found it difficult to mix the powder in well at the end, but I used a substitute powder, and maltodextrin mixes much easier.
Using glycerol is a bit more simple since it's a thick liquid. You could just pour it in by itself till you have the consistency you want, but this is not recommended due to the laxative effect. The recommended amount is 10-15% of the final concentration. Eg: 1/2 cup saline, 2 tablespoons stool, 2 tablespoons glycerol.
But again, that amount of saline is way too much. So you can follow this video demonstration where a 1:1 ratio of glycerol:stool is used, and saline is only added as needed.
When adding liquid, saline should be used instead of pure water. Saline provides osmotic protection for microbial cells, helping to maintain cell integrity and viability.
To create saline: Add 1/2 teaspoon of non-iodized salt to 1 cup of filtered or distilled water. Or 1 teaspoon of salt for 2 cups of water.
Short link to this section: HumanMicrobiome.info/FMT#treatment-length
Length is unknown and depends on the condition being treated, procedure quality, and donor quality. For IBS for example, as little as one enema has been reported to be effective for some people, but others with IBS report needing many more over many months. For the recent ASU autism study [1,2] daily oral FMTs were done over a period of 10 weeks, and gradual improvements were seen throughout, which suggests that 10 weeks or longer might be necessary in some cases. However, there were many procedure & donor deficiencies in their study, so treatment length should decrease if one is able to rectify those deficiencies.
For CDI (Clostridium difficile infection): "A single FMT was needed in 30 (52.6%) patients, 2 FMTs in 16 (28.1%) patients, 3 FMTs in 4 patients (7%), and 4–5 FMTs in 2 (3.5%) patients to achieve clinical cure" (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479393/
Sep 2020 (n=54) study showing 2 consecutive FMTs achieves 98% cure rate for C. diff, and cites other studies showing more FMTs = higher cure rates: https://link.springer.com/article/10.1007/s40121-020-00339-w
"periodic and repetitive FMT is required for a sustained effect" (Jul 2021, n=227) https://www.frontiersin.org/articles/10.3389/fmed.2021.710452/full Long-Term Follow-Up Results of Fecal Microbiota Transplantation for Irritable Bowel Syndrome: A Single-Center, Retrospective Study.
Upper vs lower routes:
Short link to this section: HumanMicrobiome.info/FMT#upper-vs-lower-routes
Upper, also known as "top down" or oral, can be done directly, or via capsules, or via naso tube.
Lower/rectal route, can be done via retention enema, or colonoscopy.
Lower-only is possibly inferior to capsules because it only gets the colon, which is a small part of the entire digestive system.
With the oral route, some people have concerns about stomach acids & bile killing off sensitive microbes before they make it to the colon. But this study showed that for c.diff, capsules are equally effective as colonoscopy procedures. The 2017 ASU Autism study also tested both routes and found no difference. Personally I found that with certain donors it seemed that rectal was more effective. And others have reported that oral seemed more effective for them. So I would recommend trying both routes if possible. I later experimented with upper vs lower and discovered that lower-only was incomplete.
March 2019 review of FMT for c.diff in Germany shows that lower route alone has a higher cure rate than upper alone, both together have the highest cure rate, but the results were confounded by age so the authors think there's no difference. Chart image.
March 2020 systematic review of c. diff concludes FMT cure rates were colonoscopy 94.8%, capsule 92.1%, enema 87.2%, and NGT/NDT (naso tube) 78.1%.
Lower endoscopic delivery of freeze-dried intestinal microbiota results in more rapid and efficient engraftment than oral administration (Feb 2021, 27 c. diff patients) https://www.nature.com/articles/s41598-021-84152-6
March 2021 study showed that both routes together was more effective than either-or: https://www.dldjournalonline.com/article/S1590-8658(21)00090-6/fulltext
For worries about SIBO see the SIBO section below.
Short link to this section: HumanMicrobiome.info/FMT#regarding-safety
Aug 2021: Systematic review with meta-analysis: encapsulated faecal microbiota transplantation – evidence for clinical efficacy https://journals.sagepub.com/doi/10.1177/17562848211041004 "Encapsulated FMT is an effective and safe treatment of rCDI, with cure rates comparable to FMT delivered through other routes"
Jan 2021: Oral Fecal Microbiota Transplant Capsules Are Safe and Effective for Recurrent Clostridioides difficile Infection A Systematic Review and Meta-Analysis https://journals.lww.com/jcge/Abstract/9000/Oral_Fecal_Microbiota_Transplant_Capsules_Are_Safe.97483.aspx "We found an overall efficacy of 82.1% with a low rate of serious adverse events"
Nov 2020: Systematic review: the global incidence of faecal microbiota transplantation‐related adverse events from 2000 to 2020 https://onlinelibrary.wiley.com/doi/abs/10.1111/apt.16148
Oct 2020: AGA releases largest real-world report on safety and effectiveness of fecal microbiota transplantation (FMT). Ninety percent of patients tracked in the AGA FMT National Registry were cured of C. difficile infection with few serious side effects. https://gastro.org/press-releases/aga-releases-largest-real-world-report-on-safety-and-effectiveness-of-fecal-microbiota-transplantation-fmt-2/
Jan 2019: Systematic review with meta‐analysis: review of donor features, procedures and outcomes in 168 clinical studies of faecal microbiota transplantation. https://onlinelibrary.wiley.com/doi/abs/10.1111/apt.15116 "Overall adverse event incidence was <1%, mostly GI‐related. Adverse event rates did not differ significantly between routes of FMT administration or indication"
Adverse events in faecal microbiota transplant: a review of the literature (2015): https://doi.org/10.1016/j.jhin.2015.10.024
The long-term effects of faecal microbiota transplantation for gastrointestinal symptoms and general health in patients with recurrent Clostridium difficile infection (2017): http://onlinelibrary.wiley.com/doi/10.1111/apt.14443/abstract
Two documented cases of aspiration pneumonia occurred during upper gastrointestinal tract endoscopy. Similar cases have been identified by other authors (Mar 2019): https://journals.sagepub.com/doi/10.1177/2050640619839918
For IBD: "My colleagues and I have now followed a small number of IBD patients undergoing FMT over 4 years, and no safety concerns have been found" http://www.gastroenterologyandhepatology.net/archives/may-2018/update-on-fecal-microbiota-transplantation-in-patients-with-inflammatory-bowel-disease/
Long-term impact of fecal transplantation in healthy volunteers (Dec 2019, n=3, 15 capsules 2 days in a row, 22g total) "one volunteer developed a fever (102F), with shivers and tachycardia of 102 per minute on the day after administration" https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1689-y - Comment/opinion: these are very common symptoms after FMT, and not a major concern.
Before the procedure:
Short link to this section: HumanMicrobiome.info/FMT#before-the-procedure
Depending on the condition, it might be helpful to take an antibiotic (for at least a few days, but they're typically prescribed for 1-2 weeks I believe) in order to clear out the current microbes so the new ones have an easier time to establish themselves . But the evidence for most conditions isn't here yet, and antibiotics can have a wide range of severe, long term detriments, and there's quite a bit of other evidence below showing antibiotics before hand are no better or worse.
This 2010 rat study showed antibiotic intake prior to transplantation did not increase establishment of the donor phylotypes. It also showed that broad-spectrum antibiotics do not "wipe everything out, letting you start from a clean slate", but rather antibiotics only temporarily suppress certain types and allow others to increase, but after antibiotic cessation, bacterial load generally returns to the previous state, but diversity seems reduced for the long-term, including after FMT. . - "Clearly, host factors also play a role in shaping the intestinal microbial ecosystem, and those factors might be also altered by antibiotic intake". "Although this finding makes ecological sense, that antibiotics might be almost as deleterious to the input community as to the endogenous community, is a highly counterintuitive result that should be taken into account in designing future bacteriotherapy protocols".
Another 2019 mouse study (STAMP) showed 3 out of 4 antibiotics (vancomycin, cefotaxime, metronidazole) used for pre-treatment "resulted in impaired transplantation efficacies". The 4th antibiotic (polymyxin) seemed to help colonization: https://www.nature.com/articles/s41467-019-09267-x
"A recent multicenter study of ulcerative colitis patients who received SER-287 (Seres Therapeutics), a microbiome-based therapy composed of a consortium of bacterial spores. Initially, the trial used vancomycin pretreatment, but the researchers found that it did not improve the results" (Jan 2019): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423689/
"specific preparation of the patient using an antibiotic induction regimen, proton pump inhibitors, or antimotility agents did not impact treatment success" (Mar 2019) https://journals.sagepub.com/doi/10.1177/2050640619839918
"Pre-treatment with antibiotics before FMT did not have any significant impact on clinical outcomes in IBS-D patients" (June 2019) https://www.healio.com/gastroenterology/irritable-bowel-syndrome/news/online/%7Bb7ea1e6f-d12c-4b81-9978-2f0d64528018%7D/oral-fmt-with-antibiotics-does-not-improve-symptoms-of-ibs-d
Pretreated with ciprofloxacin and metronidazole for 7 days and still got poor results for UC (Jul 2021) https://bmcgastroenterol.biomedcentral.com/articles/10.1186/s12876-021-01856-9
Study in C. Diff patients using FMT as first-line treatment (instead of only after antibiotics fail) gets 98% cure rate (Sep 2020, n=54): https://archive.vn/QtQlM. It cites this 2012 pooled analysis (n=289) that found no difference in cure rates between antibiotic-treated or non-antibiotic.
If you take antibiotics prior you are making a bet that the donor will restore everything the antibiotic kills off/damages. Two mouse studies showed FMT may not completely restore immune function damaged by antibiotics: (2017), (2018). And this May 2020 mouse study showed that FMT was unable to reverse the microbiome changes from antibiotics - "we found that control mice did not restore disturbed microbial communities. Instead, mice with disturbed microbial communities induced disturbance in control mice"
Neither Xifaxan nor Flagyl made low quality donors more effective for me. Certain conditions where a pathogen is a primary cause are more likely to benefit from antibiotics, but due to severe limitations in testing it can be hard to identify those individuals and the right antibiotic for them.
A clinic in Melbourne Australia gives Xifaxan 500mg 2x/day for 10 days, Flagyl 400mg 3x/day for 10 days, Nystatin 1 capsule 3x/day for 10 days prior to their FMTs: http://www.melbournefmt.com.au/fmt_procedure.html
Xifaxan only targets the small intestine  and thus may not be a good choice for cases of dysbiosis that are primarily in the colon. 2017 study showed little to no benefits from Rifaximin pre-treatment for UC .
I read somewhere that a Florida clinic (RDS Infusions) is giving Neomycin for 7 days prior to FMT.
You could also consider doing a colonoscopy prep/bowel cleanse , which includes inducing diarrhea to clear out the entire intestinal tract, but personal experience says it's not more effective than just doing the retention enema after a regular BM. Though inducing diarrhea itself may have some impacts, good or bad .
Taking a bunch of multi-strain probiotics prior did not seem to have any benefits for me, and this 2018 study showed it can result in an increased rate of infection .
PPI (proton pump inhibitor) use is not currently supported .
Short link to this section: HumanMicrobiome.info/FMT#Diet
Diet's role in FMT is not established.
A few studies showed that FMT success depends on changing bile acid metabolism , and this is very much inline with my own experiences. I did not notice a major impact from donor diet, except for when one donor ate nuts (which harm me), they were not fully digested and the undigested nuts in their stool also harmed me.
This 2017 article says OpenBiome said "their own data suggests that diet has no bearing on whether or not an otherwise healthy individual gives successful stool". The data was presented at this DDW conference then published in this abstract.
A small 2016 study concluded that patients with successful FMT for c. diff reported higher fruit and fiber consumption, compared to patients who's FMTs failed.
Sep 2021 study with 8 donors and 62 patients found no impact from donor diet.
Prebiotics and other whole foods that harmed me prior to FMT also harmed me during & after FMT with ineffective donors. I do not think you should/can introduce previously unsafe foods until you're pretty much in complete recovery. My experience with this says it is misguided to try and feed the new bacteria from the FMT. In my opinion you should continue to eat the foods which were the most beneficial to you prior to FMT. And also be aware that low quality donors may cause you to develop new food intolerances.
In cases of bile acid metabolism issues (which can cause a variety of things including diarrhea), a certain donor was able to correct/greatly improve that without doing anything special, when other donors had no impact. Donor quality seems to be the #1 factor for everything.
Short link to this section: HumanMicrobiome.info/FMT#upper-route
Using quick release capsules (Size 000 - largest possible: 1, 2) gets the entire digestive system. Using slow release (enteric coated) capsules still gets most of the small intestine and avoids stomach acids that may reduce some microbe counts.
For me it seems like with quick release methods, taking small amounts (maybe 10-15 capsules depending on their size, 1oz or less) on an empty stomach (2-3 hours after a meal and 1/2-1 hour before a meal) first thing in the morning with lots of water is more effective than taking it with a meal. Even when the meals contained prebiotics. This is likely due to various digestive system secretions triggered by meals (including large amounts of stool). Enteric coated capsules could be better but I haven't tried them, nor have I seen anyone else report doing a comparison, nor have I seen an FMT study do a comparison.
If the womb is sterile, virtually all microbes in a donor's stool would have gotten into the donor's colon via the donor's mouth first. So microbes that are evolved to thrive in the human gut should be able to make the trip from the mouth to the colon. Most probiotics are not human-sourced/human-native, so studies showing probiotics not making it through stomach acid aren't reliable for this.
Size 00 enteric coated: https://www.amazon.com/gp/product/B06XGM6HK9
Optionally, you can use two sizes of capsules and double encapsulate.
Donor poops in ziplock bag:
Then ASAP (within a few minutes) the stool is put in capsules, and capsules are swallowed. Using a 60ml Catheter Tip Disposable Syringe like this might be the easiest way:
You can shovel the whole stool into the syringe without any dilution. You may have to cut off the tip of the syringe and widen the opening by stretching the plastic with a sterilized nail. And keep some toothpicks on hand to unclog the opening.
Without the syringe, a toothpick used as a shovel worked fine for me (about 20 capsules in ~10 minutes). A fast food straw split down the middle making a canoe shape might be a little better.
Another method is to snip the corner off the bag:
And squeeze into capsules similar to how you apply frosting:
Local stores (arts & crafts like Michaels, supermarkets, etc.) should have icing piping nozzles of various sizes that would work well.
That's a two handed method that might require a capsule holding tray. Make sure the tray matches the sizes of your capsules.
If you can stomach it you can skip the capsules and just straight up swallow the poop. As a plus side, unhealthy poop is going to be a lot more repugnant (some support: ). And if the stool is too soft it will melt the capsules anyway. You could also add water and use a straw to drink the liquid.
In ASU's 2017 FMT Autism study they used the oral route (filtered out most of the large particles so no taste, and then mixed it in with liquids that the patients drank) in some patients and rectal in others and found no differences.
Short link to this section: HumanMicrobiome.info/FMT#lower-route
Donor poops in ziplock bag
Distilled/filtered water or saline is added, mix gently (so as not to oxygenate the liquid) with hand from outside the bag, do enema (enema, not colonoscopy - IE: only needs to go in an inch or so) with wide-ended (so stool doesn't get stuck) turkey baster:
I use the knee-chest position in the shower:
One hand to hold the bag under me, and the other using the turkey baster. Then shower and get into position:
Look at an image of the colon:
to understand which positions to lie in afterwards to get it to flow through the whole colon. Adding enough water to make it flow easy is important. The more you can elevate your hips at first, the better. An inversion table works great. You can also do yoga-type positions like this:
And/or decline yourself off a couch/bed with your hips on the bed and your head on the floor.
Try to hold it in overnight. You can use Imodium if needed. Once you get the liquid to the right side of the colon you can stay in that position overnight or slowly revert to a standing position and from there gravity should keep the liquid at the cecum. If you keep it there it should reabsorb.
Regarding how long to stay in each position while trying to get it to flow through the whole colon depends on a variety of factors, including how much liquid you've added, whether your colon is cleared out, whether a person has structural defects in their colon, etc.. I generally do the retention enema after a BM (when colon is most empty) and use about 3-5 turkey basters worth (each baster is about 1.75 fl oz/52ml) and can feel the liquid flow into the top of my colon within ~10 seconds of being up side down on inversion table. In other cases you might have to lie on each side for ~15 minutes.
Why you should use a turkey baster + gravity instead of other methods with tubes:
- This method is more simple and quick. No need for more complicated ones.
- It gets the entire colon. If you use tubing methods without gravity you will not get the whole colon.
- Tubes are an unnecessary risk (scraping, perforation, detachment).
- Smaller width = gets blocked/clogged easier, and thus requires filtering/straining which simply takes up unnecessary time and increases oxygen exposure.
Short link to this section: HumanMicrobiome.info/FMT#scientific-info
Review, Jan 2015: Wide benefit from fecal transplants http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284325/
Review, Mar 2016: Fecal Microbiota Transplantation: Current Applications, Effectiveness, and Future Perspectives https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895930/
Review, Mar 2017: Novel Indications for Fecal Microbial Transplantation: Update and Review of the Literature https://link.springer.com/article/10.1007%2Fs10620-017-4535-9
Review, Jan 2021: Fecal Microbiota Transplantation: A New Therapeutic Attempt from the Gut to the Brain https://www.hindawi.com/journals/grp/2021/6699268/
Guidelines: European consensus conference on faecal microbiota transplantation in clinical practice (2017): http://gut.bmj.com/content/66/4/569
Guidelines: The use of faecal microbiota transplant as treatment for recurrent or refractory Clostridium difficile infection and other potential indications: joint British Society of Gastroenterology (BSG) and Healthcare Infection Society (HIS) guidelines (2018): http://dx.doi.org/10.1136/gutjnl-2018-316818
Review, Feb 2019: Clinical Review on the Utility of Fecal Microbiota Transplantation in Immunocompromised Patients. Present literature weighs in favor of FMT in immunocompromised patients, with an acceptable adverse effect profile and minimal risk of infectious adverse events: https://link.springer.com/article/10.1007%2Fs11894-019-0677-6
Review, Mar 2019: Adjunctive fecal microbiota transplantation in supportive oncology: Emerging indications and considerations in immunocompromised patients: https://www.ebiomedicine.com/article/S2352-3964(19)30215-4/fulltext "Limited available evidence supports the notion that it is a safe therapy in immunocompetent individuals, however further studies are required"
Review, Jan 2019: Systematic review with meta‐analysis: review of donor features, procedures and outcomes in 168 clinical studies of faecal microbiota transplantation: https://onlinelibrary.wiley.com/doi/abs/10.1111/apt.15116
Short link to this section: HumanMicrobiome.info/FMT#methodologyprocedure
FMT methodology has major impact. FMT with patient's own feces results in significant changes. Possibly due to exposure to aerobic conditions. High rate of adverse events in this group: http://www.gastrojournal.org/article/S0016-5085%2815%2901581-4/fulltext
Breakthrough study that took us from being able to culture only 1% of gut microbes to >70% did so by adhering to strict anaerobic conditions: http://www.the-scientist.com/?articles.view/articleNo/46019/title/Most-Gut-Microbes-Can-Be-Cultured – http://www.nature.com/nature/journal/v533/n7604/full/nature17645.html
Oxygen tolerance of anaerobic bacteria isolated from human feces (1989): "Thirty O2-intolerant bacterial strains that reached 100% mortality after 120 min of air exposure were isolated. Ten of these strains were tested for their atmospheric O2 sensitivity as a function of air exposure time; all tested microorganisms showed a similar mortality trend on exposure to air. In fact, 50% of cells survive, on the average, after 4–5 min of atmospheric O2; this percentage decreases to 3–5% after only 20 min, and after 40 min only one cell in a thousand survives; all strains reached 100% mortality in a time range of 100–120 min." https://link.springer.com/article/10.1007/BF01568901
2017 OpenBiome/MIT study confirms that oxygen exposure is the most important factor to reduce during processing: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170922
Contradictory 2018 result from Japanese researchers showing Anaerobic Stool Preparation Method for Fecal Microbiota Transplantation Is Not Superior to Conventional Aerobic Method in Preserving Anaerobic Bacteria: https://journals.lww.com/ajg/Fulltext/2018/02001/Anaerobic_Stool_Preparation_Method_for_Fecal.125.aspx
Feb 2019: https://doi.org/10.1016/j.ebiom.2019.02.023 "The practice of preparing material for faecal transplantation in ambient air profoundly affects viable microbial content, disproportionately reducing the abundance of anaerobic commensals and the capacity for biosynthesis of important anti-inflammatory metabolites. In contrast, while reducing overall levels of viable bacteria, freeze-thaw did not significantly alter viable microbiota composition." Critique of this paper.
Stool preparation under anaerobic conditions contributes to retention of obligate anaerobes: potential improvement for fecal microbiota transplantation (Oct 2021) https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-021-02325-9
Impact of Different Fecal Processing Methods (homogenization & freezing) on Assessments of Bacterial Diversity in the Human Intestine (2016): http://journal.frontiersin.org/article/10.3389/fmicb.2016.01643/full
A Guide for Ex Vivo Handling and Storage of Stool Samples Intended for Fecal Microbiota Transplantation (June 2019) https://www.nature.com/articles/s41598-019-45173-4 "if time before transformation to transplants would exceed 24 hours (up to 72), fresh samples should not be exposed to temperatures above 20 °C (68 °F), and refrigeration at 4 °C (39 °F) can be a safe solution. Removing air above collected samples was sufficient to preserve viability. Maltodextrin-trehalose containing cryoprotectants most effective. Rapid (37°C, 98.6°F) thawing > slow thaw"
"Following 72 hours of storage, faecal microbial composition was assessed by 16 S rRNA amplicon sequencing. Refrigeration was associated with no significant alteration in faecal microbiota diversity or composition" (2015) https://www.nature.com/articles/srep16350
"The bacterial diversity of the fecal flora and the total number of bacteria were significantly reduced after 8 and 24 hours at both room temperature and 4°C" https://www.sciencedirect.com/science/article/abs/pii/S0732889304001774
"Longer durations of stool processing time, up to 3 hours, does not appear to promote a pathological microbial bloom or impact donor FMT effectiveness in CDI" - (Openbiome, 2017). Confirmed in 2020, again for C. Diff, along with storage duration of up to 520 days .
Impact of time and temperature on gut microbiota and SCFA composition in stool samples (Aug 2020, n=10) https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236944 "values obtained for methanogens, L. reuteri and total bacteria are still representative even after storage for up to 48 hours at room temperature (20°C) or 4°C. The overall microbial composition and structure appeared to be influenced more by laboratory errors introduced during sample processing than by the actual effects of temperature and time. Although microbial activity was demonstrated by elevated SCFA at both 4°C and RT, SCFAs ratios were more stable over the different conditions"
2020 study with 100 patients compared Openbiome's 23g of frozen fecal preparation delivered via lower route, vs 30-50g fresh stool obtained from patient-identified donors. Openbiome had an 86% success rate compared to 100% from fresh patient stool https://link.springer.com/article/10.1007/s10620-020-06459-0
Meta Analysis and Systematic Review, Dec 2019: Proton pump inhibitor in upper gastrointestinal fecal microbiota transplant: A systematic review and analysis https://onlinelibrary.wiley.com/doi/10.1111/jgh.14958 "We did not find evidence supporting a clinically significant benefit from routine use of PPIs in FMT protocol"
4-7 size 00 non-coated gelatine capsules, with no glycerol, stored in regular freezer was still effective for c. diff after a year (Jun 2021) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213398/
Short link to this section: HumanMicrobiome.info/FMT#Freezing
Long-term storage of feces at −80 °C versus −20 °C is negligible for 16S rRNA amplicon profiling of the equine bacterial microbiome (Mar 2021, 4 years) https://peerj.com/articles/10837/ "adding to accumulating evidence indicating that standard domestic freezers are both economical and effective for microbiome research"
4-7 size 00 non-coated gelatine capsules, with no glycerol, stored in regular freezer was still effective for c. diff after a year (Jun 2021) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213398/
Interpersonal Variations in Gut Microbiota Profiles Supersedes the Effects of Differing Fecal Storage Conditions (2018) https://www.nature.com/articles/s41598-018-35843-0 "fecal samples can efficiently be stored in −20 °C freezers or in one of the presented storage buffers, without severe alterations in bacterial composition"
Effects of the long-term storage of human fecal microbiota samples collected in RNAlater (2019): https://www.nature.com/articles/s41598-018-36953-5 "Overall, long-term stool storage at -80?°C had only limited effects on the microbiota composition of human feces"
Freezing changes ratio of Firmicutes to Bacteroides: https://microbiomethod.blogspot.co.uk/2016/11/sampling-storing-and-extracting-dna.html and https://www.ijidonline.com/article/S1201-9712(18)33883-9/abstract Standardization of gut microbiota analysis: Variability in samples taken at different times from single case and the effect of the freezing the sample (2018)
Improved Preservation of Treponema pallidum and Other Bacteria by Freezing with Glycerol (1954): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1056982 - Bacteria amounts are cut in almost half after just one freezing and thawing cycle if they are without glycerol, but the amount lost is negligible if stored in a 15% glycerol suspension. Least amount of damage when rapidly frozen and rapidly thawed.
Maltodextrin and trehalose as alternatives to glycerol (Jun 2019): https://www.nature.com/articles/s41598-019-45173-4
One study showed that for c.diff: Fresh > flash freeze > lyophilised (freeze dry) (2017): http://onlinelibrary.wiley.com/doi/10.1111/apt.13969/abstract
Another showed no difference from fresh vs frozen: Simple faecal preparation and efficacy of frozen inoculum in faecal microbiota transplantation for recurrent Clostridium difficile infection – an observational cohort study (2014): https://doi.org/10.1111/apt.13009
Is frozen fecal microbiota transplantation as effective as fresh fecal microbiota transplantation in patients with recurrent or refractory Clostridium difficile infection: A meta-analysis? (2017): https://doi.org/10.1016/j.diagmicrobio.2017.05.007 - no statistical difference.
For IBD: "my colleagues and I used both fresh and frozen stool in our study, and both types seemed to work" http://www.gastroenterologyandhepatology.net/archives/may-2018/update-on-fecal-microbiota-transplantation-in-patients-with-inflammatory-bowel-disease/
Mar 2020 Systematic Review and Meta-Analysis of c. diff trials concluded no difference between fresh and frozen.
Feb 2021 study tested engraftment using a maltodextrin:trehalose cocktail mixed with human feces, transplanted to germ-free mice, and found it was better than saline-only, but still worse than fresh.
Effects of Different Treatment of Fecal Microbiota Transplantation Techniques on Treatment of Ulcerative Colitis in Rats (Jul 2021, 10% glycerol) https://www.frontiersin.org/articles/10.3389/fmicb.2021.683234/full "FMT is a feasible method for treating UC, with frozen FMT having a superior therapeutic effect than that of fresh FMT"
Short link to this section: HumanMicrobiome.info/FMT#Dose
This 2017 review comments on a C. diff study where a small dose (few capsules) was as effective as much larger doses: http://www.tandfonline.com/doi/full/10.1080/19490976.2017.1316447 - this is in line with my own experiences.
Jan 2019 Q&A/overview of IBD studies mentions that low doses were as effective or more effective than larger ones: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423689/
This 2014 review highlights one or more studies that found the opposite: efficacy was increased after increasing dose from 20 grams to 40 grams. And also says that it seemed at the time that rectal administration was more effective than oral. https://web.archive.org/web/20180802200322/https://webcache.googleusercontent.com/search?q=cache:92IjXZvAswIJ:https://www.omicsonline.org/open-access/upper-versus-lower-gastrointestinal-route-of-fecal-microbiota-transplantation-in-the-treatment-of-clostridium-difficile-infection-2161-069X-4-169.php
Purified fecal matter had lower rates of adverse events vs crude fecal matter. No significant difference in the rate of clinical response or clinical remission between the two groups of different methods. (2018): https://link.springer.com/article/10.1007%2Fs12325-018-0800-3 The Safety of Fecal Microbiota Transplantation for Crohn’s Disease: Findings from A Long-Term Study
Increasing the Dose and/or Repeating Faecal Microbiota Transplantation (FMT) Increases the Response in Patients with Irritable Bowel Syndrome (IBS) (June 2019) https://www.mdpi.com/2072-6643/11/6/1415/htm
Efficacy of faecal microbiota transplantation for patients with irritable bowel syndrome in a randomised, double-blind, placebo-controlled study (Dec 2019, n=165) https://gut.bmj.com/content/early/2019/12/18/gutjnl-2019-319630 "89% response rate in patients who received 60g FMT. The response to FMT increased with the dose (30g vs 60g). The use of a superdonor is necessary for successful FMT"
Short link to this section: HumanMicrobiome.info/FMT#Frequency
We then compared four different strategies based on the frequency of FMT over four weeks: (1) twice a week; (2) once a week; (3) two FMTs; (4) one FMT. We were able to transfer human bacteria to mice, irrespective of the strategy used. We detected human bacteria after four weeks, even if only one FMT was performed, but there was a shift of the microbiota over time. FMT twice a week for four weeks was too frequent and perturbed the stability of the newly formed ecosystem. FMT once a week appears to be the best compromise as it allowed engraftment of Faecalibacterium, and a higher diversity of bacteria belonging to the Bacteroidales order. FMT was given orally. (2018): https://www.nature.com/articles/s41598-018-25300-3
For UC/IBD: "As for the frequency of FMT administration, once a week seemed to work in our study. The efficacy was similar in the Australian studies, in which FMT was performed 5 times a week for 8 weeks. Therefore, I think that FMT definitely needs to be given more than once, probably repeatedly over at least 6 weeks, unlike with C difficile infection" http://www.gastroenterologyandhepatology.net/archives/may-2018/update-on-fecal-microbiota-transplantation-in-patients-with-inflammatory-bowel-disease/
Short link to this section: HumanMicrobiome.info/FMT#impact-factors
Donor species richness determines FMT success for IBD (2015): https://doi.org/10.1093/ecco-jcc/jjv203 | The taxonomic composition of the donor intestinal microbiota is a major factor influencing the efficacy of faecal microbiota transplantation in therapy refractory ulcerative colitis (2017): http://onlinelibrary.wiley.com/doi/10.1111/apt.14387/abstract
Irritable Bowel Syndrome-Like Symptoms Following Fecal Microbiota Transplantation: A Possible Donor-Dependent Complication (2017): https://sci-hub.se/10.1038/ajg.2016.472
One UC/IBD study showed "Stool from one donor appeared to do much better than from the others" http://www.gastroenterologyandhepatology.net/archives/may-2018/update-on-fecal-microbiota-transplantation-in-patients-with-inflammatory-bowel-disease/
Supporting evidence for young athletes being better donors:
Athletes have higher diversity, and their gut microbiome differs at the functional metabolic level. There is a direct connection with the mitochondria . Differences in professional martial arts athletes between different competition levels (Dec 2019).
2017 review, Exercise Modifies the Gut Microbiota with Positive Health Effects. Sports medicine review. Article referencing post-lyme FMT done with athlete & follow up study. Another by the same person (Peterson) discussing "poop doping".
There have been lots of articles, such as this one, on Jonathan Scheiman's Harvard lab which is studying athletes poop/gut microbiomes in regards to athletic performance. Their June 2019 study found a performance enhancing microbe (Veillonella atypica).
Fecal microbiota transplantation confers beneficial metabolic effects of diet and exercise on diet-induced obese mice. Endurance exercise and gut microbiota: A review (2017). 2020 review. Grip strength [2-4], muscle mass, strength, and reaction time as vital signs. Physical fitness is associated with better brain structure and brain functioning. Athletes have healthier brains and can better process external sounds (2019).
Vegetarians have fewer pathogens (Thai study): http://www.jmb.or.kr/journal/viewJournal.html?doi=10.4014/jmb.1603.03057
Worse inflammatory profile in omnivores than in vegetarians associates with the gut microbiota composition: https://dmsjournal.biomedcentral.com/articles/10.1186/s13098-017-0261-x
Pooled stools (multiple donors) can be more effective .
Different responses to FMT depending on age of donor (in chickens): http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163932
FUT2 genotype and secretory status are not associated with fecal microbial composition and inferred function in healthy subjects (2018): https://doi.org/10.1080/19490976.2018.1445956
FMT success may depend on changing bile acid metabolism: http://HumanMicrobiome.info/bile
Gut fungal composition of both donors and recipients: http://humanmicrobiome.info/Candida#FMT
"the host’s inflammatory immune status can limit the efficacy of microbiota-based therapeutics to treat C. difficile infection" (Feb 2021, mice) https://www.nature.com/articles/s41467-020-20793-x
Sterile fecal filtrate is effective for treating c.diff (implicating phages): https://archive.is/gY07m
May 2017, OpenBiome presented data on donor efficacy factors and pediatric access at DDW. The nonprofit stool bank shared data from a 1,413 patient cohort of fecal transplant recipients and research from 14 abstracts at Digestive Disease Week. http://www.openbiome.org/press-releases/2017/5/23/openbiome-presented-data-on-donor-efficacy-factors-and-pediatric-access-at-ddw
According to their safety publications, OpenBiome has at least 1 donor with a 100% efficacy rate after more than 100 patients, while most of their other donors seem to be around 80%. This supports the notion that donor quality is the most important factor: https://archive.fo/py71w
Short link to this section: HumanMicrobiome.info/FMT#Outcomes
Not all donor microbes colonize the recipient. Two studies in March 2019 looked at which ones colonized . Similar 2021 study looking at Long-Term (6 months) Bacterial and Fungal Dynamics. 2020 study finds 38% of FMT-driven changes were strain replacements.
Precise quantification of bacterial strains after fecal microbiota transplantation delineates long-term engraftment and explains outcomes (Sep 2021) https://www.nature.com/articles/s41564-021-00966-0
Recipient viromes resembled those of their donors for up to 12 months. Tracking individual bacteriophage colonisation revealed that engraftment of individual bacteriophages was dependent on specific donor-recipient pairings. Specifically, multiple recipients from a single donor displayed highly individualised virus colonisation patterns (2018).
Guts of Fecal Transplant Patients Resemble their Donors' (2016) http://www.hcplive.com/medical-news/guts-of-fecal-transplant-patients-resemble-their-donors - http://bmcmedicine.biomedcentral.com/articles/10.1186/s12916-016-0698-z
For IBD: "We now have 4-year data. All 9 patients who went into remission have now relapsed. All but 1 patient chose to undergo FMT again and remission was induced in all but 1 of these patients. Most patients have elected to continue undergoing FMT anywhere between once every 2 weeks to once a month, and most are still in remission with this approach." http://www.gastroenterologyandhepatology.net/archives/may-2018/update-on-fecal-microbiota-transplantation-in-patients-with-inflammatory-bowel-disease/
Intestinal microbiota could transfer host Gut characteristics from pigs to mice , but not between mice & zebrafish . "Failure of human-to-mouse FMT to recapitulate the precise microbial contents of the human donors" https://sci-hub.se/https://www.nature.com/articles/s41435-018-0024-1
FMT can transfer not only microbiota but also the donors’ intestinal innate immune status and improved intestinal integrity: http://www.nature.com/articles/srep38599 | But may not completely restore immune function damaged by antibiotics (2017): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395657/
"By profiling bacteria coated with IgA, we identified IgA-coated bacteria associated with inflammation, and we found that microbial interactions with the host immune system can be transferred across people. This transfer of immune function is likely critical for gut microbiome therapeutics for immune-related diseases." (May 2019) https://www.biorxiv.org/content/10.1101/649384v1.full. Dynamic colonization of microbes and their functions after fecal microbiota transplantation for inflammatory bowel disease.
Fecal Calprotectin and Fecal Indole Predicts Outcome of Fecal Microbiota Transplantation in Subjects with Recurrent Clostridium difficile Infection (Mar 2019): https://www.sciencedirect.com/science/article/pii/S1075996419300459 "low calprotectin and high indole is associated with successful FMT in RCDI in both IBD positive and negative patients. We suggest that the ratio of calprotectin/indole should be evaluated for predicting outcome in FMT in RCDI"
Distinct Fecal and Plasma Metabolites in Children with Autism Spectrum Disorders and Their Modulation after Microbiota Transfer Therapy (Oct 2020) https://msphere.asm.org/content/5/5/e00314-20 "Our results provide evidence that changes in metabolites are one mechanism of the gut-brain connection mediated by the gut microbiota and offer plausible clinical evidence for a promising autism treatment and biomarkers"
Short link to this section: HumanMicrobiome.info/FMT#Autologous
FMT with patient's own feces results in significant changes. Possibly due to exposure to aerobic conditions. High rate of adverse events in this group (2015): http://www.gastrojournal.org/article/S0016-5085%2815%2901581-4/fulltext
Review, Feb 2019: Transforming medicine with the microbiome https://archive.fo/IuuPB#selection-1981.0-1997.1 "as microbiomes from individuals who may appear healthy could harbor causal factors of the condition to be treated, resulting in unforeseen resurgence of the disease" http://stm.sciencemag.org/content/11/477/eaaw1815
Review, Jun 2020: Autologous fecal microbiota transplantation for the treatment of inflammatory bowel disease: https://www.sciencedirect.com/science/article/pii/S1931524420301110
Allogenic Fecal Microbiota Transplantation in Patients With Nonalcoholic Fatty Liver Disease Improves Abnormal Small Intestinal Permeability A Randomized Control Trial (May 2020, n=21) https://journals.lww.com/ajg/Abstract/2020/07000/Allogenic_Fecal_Microbiota_Transplantation_in.19.aspx - allogenic (n = 15) or autologous (n = 6). Significant reduction in small intestinal permeability 6 weeks after allogenic FMT. Forty-six potential donors were screened to find 3 suitable donors for this study.
Autologous FMT, collected during the weight loss phase and administrated in the regain phase, might preserve weight loss and glycemic control. (Aug 2020, n=90) Effects of Diet-Modulated Autologous Fecal Microbiota Transplantation on Weight Regain https://archive.vn/WXdFd - no adverse events.
Regarding freezing your own stool before antibiotics then using it for auto-FMT afterwards, see the FAQ: http://HumanMicrobiome.info/FAQ#question-2
General FMT articles & videos:
Short link to this section: HumanMicrobiome.info/FMT#general-fmt-articles--videos
Article covering OpenBiome stool bank, one of their donors, and the need for more donors: http://news.wgbh.org/2017/04/13/science-and-technology/why-central-square-stool-bank-paying-40-poop-donation
Video covering one of OpenBiome's donors: http://www.cnn.com/videos/health/2015/08/25/fecal-transplant-poop-medicine.cnn
VICE coverage of FMT and OpenBiome: https://news.vice.com/story/your-poop-could-save-someones-life
Video coverage of Florida FMT clinic & one of their donors: https://www.youtube.com/watch?v=BxtjWynqW7A
Article covering a clinic in Tampa Florida that helps people do FMT for conditions other than c.diff. They also cover the ASU autism study, safety, and more (Jun 2017): https://www.buzzfeed.com/nidhisubbaraman/inside-tampa-fecal-transplant-clinic
The poo panacea: inside the strange, surprising world of faecal transplants. When treating antibiotic-resistant infections, injecting patients with other people’s excrement can be highly effective. Could it be the answer to dementia, anorexia and obesity too? (Jan 2020) https://www.theguardian.com/lifeandstyle/2020/jan/22/the-poo-panacea-inside-the-strange-surprising-world-of-faecal-transplants
Fecal Transplants May Be a Miracle Cure for Some of Our Nastiest Illnesses (Nov 2016): https://www.wired.com/2016/11/microbiome-therapy-making-fecal-transplants-better/
There is growing evidence faecal transplants could be causing some patients to take on the physical and mental traits of their donors, including body shape and even symptoms of depression (2017): http://www.abc.net.au/news/2017-05-09/faecal-transplant-side-effects-explored-at-conference/8510270
Doctors transfer the power of gut bacteria (Oct 2016): http://azdailysun.com/news/local/doctors-transfer-the-power-of-gut-bacteria/article_5956de37-7b12-52ab-9491-0853b067f39c.html
2017 study shows FMT helpful for Autism: https://medicalxpress.com/news/2017-01-gut-microbe-potential-treatment-autism.html
Article's covering researcher Jeff Leach's microbiome experiments (including DIY FMT) with the Hadza tribe: http://www.popsci.com/article/science/scientist-gives-himself-fecal-transplant-try-hunter-gatherers-microbiome - http://humanfoodproject.com/rebecoming-human-happened-day-replaced-99-genes-body-hunter-gatherer/ - http://www.rawstory.com/2017/07/heres-what-happened-to-a-professor-of-genetic-epidemiologys-gut-microbiome-when-he-lived-with-hunter-gatherers/
Article covering researcher Lauren Petersen, who cured herself of post-lyme illness with DIY FMT: http://mobile.the-scientist.com/article/49450/athletes-microbiomes-differ-from-nonathletes
Guy does DIY FMT for IBS (does some extreme & unnecessary things): http://www.theverge.com/2016/5/4/11581994/fmt-fecal-matter-transplant-josiah-zayner-microbiome-ibs-c-diff
Thepowerofpoop.com has lots of good & useful info. But also some wrong/outdated info. The blending part of their instructions should be avoided. You don't want to do this since it will kill off a lot of anaerobic microbes (the gut is an anaerobic environment!). And their warning about capsules & SIBO is wrong (see the SIBO section on this page). Haven't vetted their whole site. Looks like the site went down early 2019. You can use archive.org to see old pages.
Short link to this section: HumanMicrobiome.info/FMT#SIBO
Some claim using quick release capsules can cause SIBO . But it's more likely due to inadequate donor screening/selection. Top-down methods that empty into the stomach or small intestine are common/standard in all types of official and experimental FMT practice , and there is no evidence that they cause or worsen "SIBO".
Feb 2021 study showing FMT via capsules can treat SIBO: https://bmcgastroenterol.biomedcentral.com/articles/10.1186/s12876-021-01630-x
In ASU's 2017 FMT Autism study they used the oral route (filtered out most of the large particles so no taste, and then mixed it in with liquids that the patients drank) in some patients and rectal in others and found no differences.
This case seems to prove SIBO is a donor problem rather than a "quick release capsule" problem: https://archive.is/ixi6Y - recipient develops SIBO from colon-only FMT.
Naso tube FMT study for IBS symptoms and predominant abdominal bloating see significant improvements https://www.gastrojournal.org/article/S0016-5085(18)30860-6/abstract Fecal Microbiota Transplantation in Irritable Bowel Syndrome with Predominant Abdominal Bloating: Results from a Double Blind, Placebo-Controlled Clinical Trial
Review of 97 patients finds no difference between top and lower routes (2018): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6305536/
Systematic review with meta‐analysis: review of donor features, procedures and outcomes in 168 clinical studies of faecal microbiota transplantation (Jan 2019) https://onlinelibrary.wiley.com/doi/abs/10.1111/apt.15116 "Overall adverse event incidence was <1%, mostly GI‐related. Adverse event rates did not differ significantly between routes of FMT administration or indication"
Review of 150 patients finds "Small bowel exposure to donor stool was not related to symptoms" (Oct 2019): https://www.ncbi.nlm.nih.gov/pubmed/30882536
Myself and this person had no problems from DIY oral FMT. And I did it many times from multiple donors. I also experimented with lower vs upper route and lower route only does not seem to be complete.
SIBO - valid term or misnomer based on incorrect understanding of the gut microbiome? I argue the term should be replaced with simply "dysbiosis". https://medium.com/@MaximilianKohler/sibo-valid-term-or-misnomer-based-on-incorrect-understanding-of-the-gut-microbiome-f26c74be141b
Short link to this section: HumanMicrobiome.info/FMT#fmt-clinics
- There is no official source of FMT that can be blindly trusted. Including stool banks, clinical trials, hospitals, etc.. All official sources of FMT have had significant deficits in donor quality for many years . None provide proof of donor quality, and the clinics do not track and report results. You must learn what it means to be a high quality donor and verify that the provided donor is high quality. If they will not verify that information with you, do not use them.
Here is a list of questions you should be asking of every stool provider: https://archive.ph/FAtNo#selection-1233.0-1233.1
There are a large amount of clinics/doctors that provide stool and/or do the procedure themselves for c.diff:
Providers & trials (US & worldwide): https://web.archive.org/web/20191222020326/http://thefecaltransplantfoundation.org/providers-trials - site seems to be down in 2020, but archive link works.
List of doctors in the US who do FMT: https://mygijourney.org/c-diff-help/
https://web.archive.org/web/20170515194927/http://thepowerofpoop.com/epatients/where-to-get-fecal-transplant - some entries are outdated/undependable/no longer offering assistance with FMT.
OpenBiome (stool bank - c.diff only. You can use their site to find doctors): http://slice.mit.edu/2015/10/29/podcast-gross-science/ – http://www.openbiome.org/ | Analysis of OpenBiome's safety and efficacy (2018): https://archive.vn/xkQGU
Below are the clinics who do FMT for things other than c.diff, and the info I have on them:
There are a variety around the world who do FMT themselves or refer you to tested donors. Most charge thousands of dollars and there are multiple reports of people paying that money and getting poor results or even being harmed. There is no relation between their cost and their quality. When I contact these clinics to try and get information to evaluate whether they're worth the money, they typically curtly decline. It seems they have more than enough desperate people coming with no questions asked that they don't bother with people who want answers first.
Most of these clinics do not have enough info on their sites for knowledgeable people to be able to vet their donors & procedures. For example, we need the kind of info in the screening questionnaire above for each clinic's donors. Do not spend thousands of dollars without them proving to you their donors & procedure are high quality!! In my experience, and from feedback from others, high quality donors are young (ideally under 25), athletic, 0 lifetime antibiotic usage, and have identical type 2/3 (NOT 4), dark, small, firm & dry stools.
When we do get to see more info like this video coverage of one of OpenBiome's donors we can see numerous problems, such as very low quality (type 5) stool, and a donor who in my opinion is not visibly in perfect health: http://www.cnn.com/videos/health/2015/08/25/fecal-transplant-poop-medicine.cnn
One of the most important things is actually seeing the whole stool, prior to processing. Otherwise you have no idea what the quality of the stool they are using is. Getting video/image coverage of the donors is also very helpful/important.
It seems that currently (Sept 2018) none of the major clinics in the US, AU, or UK have a good understanding of what makes a high quality donor, nor do they bother to collect enough data to determine the impact (safety & efficacy) of each donor.
Some of these clinics seem to be over-processing the stool. I have not seen any data suggesting this is needed/beneficial. And to the contrary there is data suggesting that there are a wide variety of beneficial organisms and compounds in whole stool , and processing can lead to detrimental exposures like oxygen. Many people have reported good results from DIY at home, and those are with zero processing. Thus my current opinion is that fresh, whole stool containing all microbes and microbial byproducts in their natural environment is the best.
I created this sub for people to share their experiences: https://old.reddit.com/r/FMTClinics
I started up HumanMicrobes.org in late 2020 due to the significant deficiencies covered in this wiki section.
Cost comparison of a few of the main clinics: http://www.ibsgroup.org/forums/topic/331018-taymount-clinic-vs-newbery-clinic-for-my-fmt/
Discussion here on possible reasons for poor results from the clinics and OpenBiome: http://www.ibsgroup.org/forums/topic/341042-fmt-at-taymount-clinic/page-2#entry1397794
More info on learning what donor quality means and why it's so hard to find high quality donors: https://archive.fo/I3wSb#selection-1883.59-1883.60
The following list is an incomplete work in progress.
Taymount Clinic (multiple locations - UK, Bahamas):
Short link to this section: HumanMicrobiome.info/FMT#taymount-clinic-multiple-locations---uk-bahamas
After many people reported detrimental outcomes at Taymount they started making patients sign an NDA, agreeing not to publish any comments on social media without Taymount's approval: https://web.archive.org/web/20200814155507/https://taymount.com/taymount-docs/QM090-03-Client-Agreement.pdf. There have also been numerous reports of patients being sent legal threats by Taymount after reporting poor outcomes at the clinic.
Procedure details: https://www.youtube.com/watch?v=HxgME7hbnJc&t=57m30s
12/09/2018: Some very useful info about Taymount's donors: https://archive.fo/UtDaL - pretty much confirms all my concerns about their donors. There's a 0 percent chance all those people are safe and effective donors. That many donors coming and going certainly helps to explain some of the widely varying results people get from them. And Taymount's protocol of using a different donor each day for 10 days doesn't allow them to know which donors are safe or effective. It also gives us a peek at their questionnaire, which of course is also a joke - "no chemotherapy in last 3 months". My god.
This July 2019 publication gives additional info/confirmation about donor screening/criteria: https://www.sciencedirect.com/science/article/pii/S2452231719300077
Sep 2020 case report for Multiple Sclerosis https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506051/
They do FMT via enema only. Prep is colon cleansing via magnesium oxide salts, then colon hydrotherapy. They use the technique that replaces air with nitrogen when filtering the microbes during donor stool prep. They homogenize & filter out all the food almost down to microbe level size. They use a centrifuge to separate microbes. Then freeze.
In this video they refer to the process as "the full 10 days": https://www.youtube.com/watch?v=DTIPWFysINA - this is likely way too short a time period for many/most people/conditions.
Taymount's standard procedure is to do 1 transplant from a different donor each day for 10 days over 2 weeks. In theory getting 10 different donors has its benefits, but only if they're all high quality. And current reports are poor and suggest they are not. Using this method there is no way for them to determine which donors are having what impacts. It doesn't allow them to judge the safety and efficacy of each donor.
Adrienne Grierson, who is making a film on FMT, says that both Taymount locations use the same donors, and the stool is shipped to the Bahamas from the UK, but there is no verification of this on their site. 11/25/2017
5/13/2018: Taymount announces (via facebook) that they're opening a new location in Canada, and they'll be using frozen stool shipped from the UK location (so same donors).
Taymount says lots of nice things about their donors that most lay people find very convincing, but I haven't seen any strong evidence that their donors are high quality. We need to see the stool types and the questionnaire info for the donors. Video coverage of donors is very helpful. 11/25/2017
01/15/2017 Taymount declined to allow patients to verify donor quality prior to committing to thousands of dollars for treatment.
Here's an example of the kind of clueless, deceptive/highly biased type of person employed by Taymount: https://web.archive.org/web/20190914224910/https://www.pnwnutrition.co/single-post/2019/09/12/FMT-Troubleshooting---Going-beyond-the-gut - this article is full of ignorance and misinformation (which is thoroughly debunked throughout this wiki), and topped off with this ludicrous, unsupported statement at the end: "The implants that patients receive at our clinic contain an ideal balance of everything that makes up a healthy gut microbiome".
Dove Clinic, UK
Short link to this section: HumanMicrobiome.info/FMT#dove-clinic-uk
Dove clinic uses stool/donors provided by Taymount.
This is a publication by them:
A Retrospective Outcome Study of 42 Patients with Chronic Fatigue Syndrome, 30 of Whom had Irritable Bowel Syndrome. Half were treated with oral approaches, and half were treated with Faecal Microbiome Transplantation (July 2019) https://www.sciencedirect.com/science/article/pii/S2452231719300077 "the FMT group improved to a greater extent"
RDS infusions, Florida:
Short link to this section: HumanMicrobiome.info/FMT#rds-infusions-florida
Reportedly they've closed as of early-mid 2019. You can still order directly from their donors.
Giving Neomycin antibiotic for 7 days: http://www.ibsgroup.org/forums/topic/250274-ibs-d-started-rds-probiotic-infusion/
info (at) rdsinfusions.com
Video coverage of the clinic, one of their donors, and some of their processing methods: https://www.youtube.com/watch?v=BxtjWynqW7A
Michael Garcia, one of their donors, deals directly with people as well and has his own website: https://archive.fo/qGpMx
Their other known donor is "AR" .
For anything other than c.diff they will not do the FMT, but will refer you to their tested donors. 11/25/2017
Probably the most affordable clinic: $80 per infusion and $10 per capsule. Dry ice shipping is the most expensive part - $160. The infusions seem more cost-effective for the amount you get.
I emailed them about their procedure & donor quality and they wouldn't provide info without paying for a consult ($300) 11/25/2017. And even after the consult I didn't feel that most of my questions were answered.
From the Buzzfeed article it seems like they have one or more low quality donors, and possibly one or more high quality donors.
This interview has some info: https://web.archive.org/web/20140329071536/http://thepowerofpoop.com/interview-with-fecal-transplant-doctor/ - I found their stated donor criteria laughable.
12/18/2017: Horrible experience, and other info: https://archive.is/SSk2i
Melbourne FMT, Australia:
Short link to this section: HumanMicrobiome.info/FMT#melbourne-fmt-australia
http://www.melbournefmt.com.au/donors_selection_screening.html - criteria seem weak. No mention of stool type. 11/25/2017
They give multiple antibiotics prior, for 10 days: http://www.melbournefmt.com.au/fmt_procedure.html
Queensland FMT, Australia:
Short link to this section: HumanMicrobiome.info/FMT#queensland-fmt-australia
Dr. Paul Froomes.
Mandatory antibiotics prior to the FMT.
Enema only, costing $400 per enema.
Donor criteria look poor.
Center for Digestive Diseases (CDD), Sydney, Australia:
Short link to this section: HumanMicrobiome.info/FMT#center-for-digestive-diseases-cdd-sydney-australia
Thomas Borody's (a leading FMT researcher) clinic.
Here's a detailed 2011 paper titled "Treating Clostridium difficile Infection with Fecal Microbiota Transplantation" he published, but things might have changed since then: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223289
2015 one titled Fecal microbiota transplantation: A ‘How-To’ guide for nurses https://www.collegianjournal.com/article/S1322-7696(14)00081-X/fulltext
Published papers: https://orcid.org/0000-0002-0519-4698
Donor quality/requirements seem very poor:
Donor Recruitment for Fecal Microbiota Transplantation (2015): https://doi.org/10.1097/MIB.0000000000000405
According to a patient, the cost in 2021 is $11,500. Includes Vancomycin 10 days before, colon washout, 30 enemas to do at home - recommended to start out with daily enemas for the first week, then taper off.
Short link to this section: HumanMicrobiome.info/FMT#microbiomaorg
Microbioma.org is not a clinic, but ships stool worldwide. It's run by someone in Spain. Results are not being systematically tracked and reported, and thus, like the other options, it cannot be trusted.
They make unsupported "super donor" claims, and put up other seemingly-ungenuine testimonials on their site. The sparse and brief experience reports on Facebook FMT groups have seemed 50/50 positive/negative.
I am including the following statement because I've seen people spreading baseless claims that have the potential to cause harm, and which have been the complete opposite of my experience and observations:
I worked with Microbioma for ~2 years. My personal experience with them was that they are not scientific or knowledgeable. I had the option to use their donor for free, due to the 2 years of volunteer work I did for them, but declined to do so due to the above issues. Just because someone is willing to provide advice doesn't mean that advice is sound.
2021-07-20: Microbioma.org WARNING. "Ivan Watson" alias of the person running it, has resorted to blatant lies in order to sell his product. - Archived. Direct link to album. Album link at another host.
He then tried to scrub the internet of all evidence exposing him. He got the reddit post removed https://archive.ph/tVRT0 for "posting personal information", and got the imgur links taken down (false copyright claim) https://archive.ph/FeTKU.
2021-08-03: He hired a lawyer to threaten me to take down all the information. Obviously I will not. Unfortunately, his legal threats have been successful in getting the Facebook FMT groups to censor themselves in his favor.
FMT Solution LLC, Mexico:
Short link to this section: HumanMicrobiome.info/FMT#fmt-solution-llc-mexico
Jason Klop (naturopath) seems to be the main person running this project right now. Mark Davis (naturopath) was said to be involved at some level previously.
Very very expensive.
$15,000 for 10 days of treatment. As of 2020 it's now $15,000 for 16 weeks of treatment. No info about donor quality.
12/10/2017: Not willing to help patients verify donor & procedure quality prior to the transplants. Not willing to help reduce costs in any way.
People have reported that he promised to provide them donor quality information after they paid, and then did not, even once at the FMT retreat.
Follow up article stating the B.C. Naturopathic Association (BCNA) voted to suspend Klop's membership https://www.cbc.ca/news/canada/british-columbia/bc-naturopath-fecal-transplants-autism-college-response-1.5425909
In late 2020 Jason Klop started up another similar website: https://novelbiome.com
Aug 2021: Naturopath banned from selling pills, enemas made from feces after complaint they were made in 'household lab' https://www.cbc.ca/news/canada/british-columbia/bc-naturopath-fecal-transplants-college-naturopathic-physicians-1.6159260
Dr Jorge Llamas, Mexico:
Short link to this section: HumanMicrobiome.info/FMT#dr-jorge-llamas-mexico
It was reported on facebook that this doctor treated 20 families. Only one person saw improvements. The doctor wouldn't answer questions or give evidence of donor quality. The patients had a sample of donor stool tested and said it was "lacking diversity, the bacterial count was reduced, the bacterial viability was almost null ( mostly all dead bacteria ) and the Ph was off". Permalink.
Dr. Dariusz Korolik, Poland:
400 EUR for 1 deep retention enema. Not too much info on donors or outcomes.
Steve Baskin's lastplace.org:
Short link to this section: HumanMicrobiome.info/FMT#Steve-Baskins-lastplaceorg
Another project started up in the community that initially had wide community support, but then the founder went off the rails: https://web.archive.org/web/20210501081123/https://old.reddit.com/r/HumanMicrobiome/comments/n18p6b/in_response_to_criticism_about_steve_baskins/
06/06/2021: Apparently it's now shut down shortly after it started up.
Newbery Medicine, Argentina:
Short link to this section: HumanMicrobiome.info/FMT#newbery-medicine-argentina
Newberry is closing down 4/18/2018.
"Healthy stool is mixed with saline solution and then ultra-filtered to extract everything except the microorganisms." Via rectal route only.
I emailed them about their procedure & donor quality and they wouldn't provide info. 11/25/2017
This interview has some info: https://web.archive.org/web/20161103183641/http://thepowerofpoop.com/interview-dr-silvio-najt/ - looks like they have a preference for fresh stool, which is good, but they said they're building a frozen bank. And not enough info about donor selection.
This guy is doing FMT at Newbery for Ulcerative Colitis: https://medium.com/@scottsundvor
Mediocre improvements from 5 implants: https://robbwolf.com/2017/01/13/what-happened-when-i-got-a-fecal-transplant/
FMT for IBS-D at Newbery Medicine (failure): https://web.archive.org/web/20180203231309/http://www.ibsgroup.org/forums/topic/344097-my-fmt-for-ibs-d-at-newbery-medicine/
Short link to this section: HumanMicrobiome.info/FMT#Experiences
Experiences from clinics only: https://old.reddit.com/r/FMTClinics/
Experiences on /r/fecaltransplant: https://old.reddit.com/r/fecaltransplant/search?sort=new&restrict_sr=on&q=flair%3AExperience
A variety of success stories on /r/FMT: https://www.reddit.com/r/fmt/search?sort=new&restrict_sr=on&q=flair%3ASuccess%2BStory
Success stories on thepowerofpoop.com: https://web.archive.org/web/20170201034115/http://thepowerofpoop.com/fecal-transplant-success-stories/