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Make submissions to GEO SRA

adrianas edited this page Aug 3, 2022 · 2 revisions

Here are some digested steps to submit samples to GEO/SRA. Most of the information needed is located in their FAQ

  1. Log in to https://www.ncbi.nlm.nih.gov/geo/
  2. Go to my submissions, add the lab info and then click on create new submission
  3. Fill the metadata spreadsheet with all the relevant info.
  4. Once you've gathered all the raw and processed files you want to push and are listed in the metadata spreadsheet, then you check the size of the total files. If it's bigger than their limit, contact them.
  5. Also contact them to let them know that you are creating a new GEO submission and require a private SSH key to submit the raw data into SRA (They help you put it together with the GEO).
  6. Push all the files to the repository, then when everything is uploaded, including the metadata spreadsheet, submit the request to GEO (select 3 years from now to keep it private, this can be changed later).
  7. A notification email will show up with the Accession number and the submission should be ready.
  8. To create an access token, click on reviewer access, and then a popup banner will show up just above with a link that you need to click to generate that token.

They'll provide you with credentials to access their ftp:

host address	ftp-private.ncbi.nlm.nih.gov
username	geoftp
password	********

After connecting, you must navigate to your personalized upload space, where uploads can be made:

cd uploads/[user]@uw.edu_******

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