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AFM Support

Paige Quarterman edited this page Jun 14, 2026 · 1 revision

AFM Support

Beyond electron microscopy, FermiViewer imports Bruker Nanoscope atomic force microscopy files (.spm and the numbered .000.nnn family) and treats them as fully calibrated height images — so every viewing, measurement, and export tool works on them unchanged.

AFM height map with the calibrated Z-scale color bar

What gets imported

  • Lateral calibration — x/y pixel pitch in nm, from the scan size, so distances and the scale bar are correct.
  • Height (Z) calibration — the pixel value is the physical height (nm), via Bruker's hard-scale × sensitivity chain (both the modern and legacy conventions are handled).
  • Channels — the Height/ZSensor channel opens by default; other channels (amplitude, phase, stiffness, …) are available too.

The Z-height calibration is cross-validated exactly against the pySPM reader on a real Bruker v9.3 scan.

The Z-scale color bar

AFM height is best read as false color with a calibrated scale. In the Adjust → Color range controls you can:

  • Auto-scale the color range to the data, or type fixed min/max in nm to compare scans on a common scale,
  • set a tick step for the labeled height scale, and
  • place the color bar on the left or right — it sits in its own gutter with nm tick labels, beside the lateral scale bar.

So one figure shows both length scales: the lateral scale bar (x/y) and the height color bar (z).

Measuring height

Use the Line profile tool (L) to take a height cross-section — the dock plot shows height (nm) vs distance (nm). See Measurements.

Next: Supported Formats.

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