11436200

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Bold's minimal medium recipe"

To make 1L of Bold’s, use 966mL distilled water.

	Stock solution		Bold’s
	g/L	g/200mL	mL/L
NaNO3	50	10	5
MgSO4•7H2O	10	2	5
K2HPO4	20	4	5
KH2PO4	30	6	5
CaCl2•2H2O	6.64	1.33	5
NaCl	5	1	5
	Stock solution		Bold’s
	g/L	g/100mL	mL/L
EDTA     & KOH	25 15.5	5 3.1	1
FeSO4•7H2O	2.5	0.5	1
H3BO3	5.7	1.14	1
Micronutrients (see below)			1
Micronutrients	g/100mL
Na2MoO4	0.108
ZnSO4	1.57
Co(NO3)2•6H2O	0.05
MnCl2•4H2O	0.226
CuSO4•5H2O	0.158

Agar: 15 g/L (1.5%)

Agarose pipettes: 1.5 g/L (0.15%)

Dark: 1.2 g/L Sodium acetate + 0.9 g/L agarose

Yeast extract: 2 g/L

Document generated by Confluence on May 22, 2024 11:44

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