Chlamydomonas information and protocols_11436157

TheNessLab : Chlamydomonas information and protocols

Created by Robert Ness, last modified by Jimmy Issa on Jan 31, 2023

This page contains basic information and lab protocols specific to Chlamydomonas

1. Natural Chlamydomonas reinhardtii Isolates/Strains

This describes many if not all the natural/WT strains of Chlamydomonas. Most of the information comes from ChlamyCollection.org

2. Molecular Protocols

• PCR

• Gel electrophoresis

• DNA extraction

• DNA extraction: Chelex

• DNA Extraction using Promega DNA Kit

• RNA extraction

• Quantify dsDNA concentration using Qubit

3. Culture Protocols

• Bolds Medium Recipe
• Sexual Cycle
• Freezing and thawing cultures
• TAP(-N) Recipe
• Easy single colony isolation of vegetative cells
• How to grow Chlamy quickly in TAP
• Spin-free crosses

4. Available Strains

5. Staining Protocols

• Nile red staining

6. Other Protocols

• Neutral lipid methanol-chloroform extraction (borrowed from Mike Phillips)

7. Templates

• Multi-well plate templates

8. General Resources

• Chlamydomonas Resource Center
• Chlamydomonas Reference Assembly
• Algal Annotation Tool
• See more in Table 3 of 10.1016/j.tplants.2014.05.008
• Chlamy Library