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Created by Robert Ness, last modified on May 03, 2021
The purpose of this project is to find new natural genotypes of Chlamydomonas reinhardtii and closely related Chlamydomonas species
The major hurdle is the development of a molecular, PCR-based screen for Chlamydomonas-like alge in natural sample
Culture Technique
TAP Media preparation
TAP-agar preparation
Inoculation in liquid
Inoculation on agar
Autoclaving
Sterile technique
DNA extraction
phenol-chloroform
Qubit
PCR
Gel Electrophoresis
Tasks
Test PCR primers on panel of Chlamydomonas
Design Additional primers
Develop protocol for DNA extraction from green liquid
Develop colony PCR protocol
Document generated by Confluence on May 22, 2024 11:44
Atlassian