Bold's carbenicillin plates

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title: "TheNessLab : Bold's carbenicillin plates"

1. TheNessLab
2. Chlamydomonas information and protocols

TheNessLab : Bold's carbenicillin plates

Created by Robert Ness on Dec 16, 2019

To prepare Bold's agar plates with 50 μg/mL carbenicillin:

Final concentration in plates

• 50µg / ml
• (50 * 1000 )µg per litre = 50mg carbenicillin per litre

Steps:

• Make 1L Bold's agar
• Autoclave for 15 min at 121°C
• When cooled to 45°C-50°C add 1 mL of carbenicillin stock solution (50 mg/mL)
• Pour into Petri dishes and allow to solidify. Store at 4°C.

Stock carbenicillin:

To prepare a 50 mg/mL stock solution of carbenicillin, dissolve 0.5 g of disodium carbenicillin in 9.5 mL of H2O. Top up the volume to 10 mL and filter-sterilize using a 0.22-μm filter. Store in 1-mL aliquots at −20°C.

http://cshprotocols.cshlp.org/content/2006/1/pdb.rec533.full?text_only=true

250ml

Document generated by Confluence on May 22, 2024 11:44

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