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Rob Ness edited this page Jul 3, 2024 · 1 revision

title: THEN (TheNessLab)

Space Details:


Key	THEN
Name	TheNessLab
Description
Created by	iitsadmin (Apr 25, 2017)

Available Pages:

The Ness Lab Wiki
- Chlamydomonas information and protocols
  - Bold's carbenicillin plates
  - Bold's minimal medium recipe
  - Chlamydomonas Phenol-Chloroform DNA extraction
  - Chlamydomonas RNA extraction with Tri-Reagent
  - DNA Extraction: Chelex Method
  - DNA Extraction using Promega DNA Kit
  - Easy single colony isolation of vegetative cells
  - Freezing/Thawing protocol
  - Gel electrophoresis
  - How to grow Chlamy quickly in TAP
  - Multi-well plate templates
  - Mutation Accumulation Protocol
  - Natural Isolates/Strains
  - Neutral Lipid Methanol-Chloroform Extraction
  - PCR with Broad-Range,C.reinhardtii-specific Primers
  - Protocol for the sexual cycle in C. reinhardtii
    - Protocol to cyopreserve C. reinarhdtii
  - Quantify DNA concentration using Qubit
  - RNA Extraction with Maxwell RSC Plant RNA Kit (Promega)
  - Spin-free crosses
  - Standard Chlamydomonas reinhardtii PCR
  - Strains available in the lab (cryopreserved and/or on agar slants)
    - Ness Liquid Nitrogen Dewar
  - TAP(-N) Recipe
- Coding tips and tutorials
  - Bash Reference
  - BIO362: Learn Python through bioinformatics
  - cyvcf2 tutorial
  - Installing Python Packages on the Server Without Root Access
  - Installing R Packages on the Server Without Root Access
  - Intro to Awk
  - Making figures: ggplot examples
    - Bar plot
    - Making an ideogram / karyogram
    - Theme used for ggplot figures
    - X-Y scatterplot
  - Syncing GitHub repos on hpcnode1
  - Tips & Tricks for Efficient R Work
  - Useful .bash_profile settings
- Compute Canada
- File lists
- How-to articles
  - Nile Red Staining
- HPCnode 1 Documentation
  - Basic Project Structure
  - Connecting to the server
  - Connecting to the Server Using Windows
  - File Structure of HPCnode1
  - Getting started with Conda on HPCnode1
  - Getting the VPN
  - Important Files
  - Running Jupyter Notebooks on the server
- Lab supplies and ordering
  - Ordering supplies
- Projects
  - MA Expression
  - The effect of salt-stress on MA and adaptation
  - Wild Chlamy
- Teaching and Research Students
  - BIO481 marking scheme
  - ROP Marking Scheme

Document generated by Confluence on May 22, 2024 11:44

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