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title: "TheNessLab : TAP(-N) Recipe"

1. TheNessLab
2. The Ness Lab Wiki
3. Chlamydomonas information and protocols

TheNessLab : TAP(-N) Recipe

Created by Wen Chen on Oct 30, 2018

Use the following recipe to prepare TAP medium.

To prepare 1L of TAP medium add the following solutions in order:

Order #	Solution	Amount
1	ddH2O	967 mL
2	1M Tris base (e.g. Trizma)	20 mL
3	Phosphate buffer II	1.0 mL
4	Solution A	10.0 mL
5	Hutner's trace element	1.0 mL
6	Glacial acetic acid	1.0 mL

How to prepare the components of TAP medium:

• 100 mL of Phosphate buffer II: Dissolve 10.8 g K₂HPO₄ + 5.6 g K₂H₂PO₄ in ddH₂O, then top up to 100 mL with ddH₂O. 

• 500 mL of Solution A: Dissolve 20 g NH₄Cl +5 g MgSO₄• 7H₂O + 2.5 g CaCl₂• 2H₂O in ddH₂O, then top up to 500 mL with ddH₂O.

                 ***Omit** NH₄Cl** when preparing Solution A for nitrogen-free TAP (TAP-N)

• 500 mL of 1M Tris base: Dissolve 60.57 g Tris Base in ddH₂O, then top up to 500 mL with ddH₂O.

If you do not add the solution components of TAP in order, your TAP medium might not work.

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