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mesoSPIM_V4_custom_parts
Fabian F. Voigt edited this page Feb 12, 2019
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A mesoSPIM Version 4 requires at least 14 custom parts to be made.
For the mesoSPIM, 1 left + 1 right galvo mount is required. Currently, there are two options: To use Thorlabs scanners or Scanlab scanners - choose the galvo mounts accordingly.
Image | Description | Quantity | Link to drawing |
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Galvomount-22mm-diameter-Thorlabs-Left | 1 | Link |
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Galvomount-22mm-diameter-Thorlabs-right | 1 | Link |
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Galvomount-22mm-diameter-Left-Scanlab-V4 | 1 | Link |
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Galvomount-22mm-diameter-Right-Scanlab-V4 | 1 | Link |
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Cube_30_with_35mm_hole: Qioptiq 30 mm cube, one hole has to be widened to 35 mm. | 2 | Link |
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60mm-blank-cageplate-LCP03M-Nikon-MOD: Thorlabs LCP03/M blank 60 mm cage plate, needs to be milled to create an F-mount cage plate for the scan lenses | 2 | Link |
Image | Description | Quantity | Link to drawing |
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V4_Servo-Dynamixel-Adapter-V2: Interfaces the Dynamixel servo with the zoom axis of the MVX-10 microscope- Recommended material: Brass. | 1 | Link |
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MVX-10-Bracket-V5: Allows the MVX-10 macroscope to be mounted on a X95-carrier | 1 | Link |
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X95carrier-to-M605-adapter-V1: Allows the M-605 linear stage for focusing to be mounted on an X95-carrier | 1 | Link |
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M605-to-X95-adapter-V1: Allows a FLS-95 rail for the detection system to be mounted on the M-605 focusing stage | 1 | Link |
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XT95P3_X95_Base_plate_mod: Thorlabs XT95P3 base plate, additional countersunk holes for the camera mount are required | 1 | Link |
Image | Description | Quantity | Link to drawing |
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Sample-Stage-Gate-Safety-Volume, also called the "birdhouse". This is a massive block of aluminium carries the sample stages. | 1 | Link |
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M-116-Adapter: Allows to attach posts with M6 set screws to the M-116.DG rotatio stage | 1 | Link |
Image | Description | Quantity | Link to drawing |
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Large-cuvette-mount-30: Allows a 30x30 mm cuvette to be used as an immersion cuvette. Recommended material: POM. | >1 | Link |
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Large-cuvette-mount-40: Allows a 40x40 mm cuvette to be used as an immersion cuvette. Recommended material: POM. | >1 | Link |
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Large-cuvette-mount-50: Allows a 50x50 mm cuvette to be used as an immersion cuvette. Recommended material: POM. | >1 | Link |
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Background
- mesoSPIM history
- Optical design
- Electronics
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Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
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Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants