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mesoSPIM_V5_sample_xyz_stages

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Fabian F. Voigt edited this page Mar 11, 2019 · 7 revisions

Setting up the sample XYZ stages

Overview

XYZ Stages Overview of the mesoSPIM V5 sample stages: The travel range of the V5 sample stages is currently 52 mm × 52 mm × 102 mm (X/Y/Z). The stages are produced by Physik Instrumente, Germany: L-509.20DG10 (for X & Z movement), L-509.40DG10 (Y movement), M-060.DG (sample rotation) and controlled by a C-884 controller. The sample XYZ & rotation stages are mounted on a gantry built from Qioptiq X-95 profiles.

Step-by-step guide

Build the X95 gantry

XYZ Stage gantry

Overview of a mesoSPIM V5 with the stage gantry

Please have a look at the drawings here and here for the gantry dimensions. The FLS-95 carriers below the X-95 profile that the XYZ stages are attached to are intended as safety stops to prevent the heavy stage assembly from dropping onto the rest of the microscope.

Assemble the stages

The L-509 stage can be connected to a X-95 carrier with a M6-thread pattern in a 25 mm-grid using M4-to-M6 thread adaptors and M4 screws.

For the assembly of the stages, please follow the instructions provided by PI.

Wire the stages to the controller

XYZ Stages

Cable assignment to channels is as follows:

  • 1st axis: L-509.20DG10 ("X")
  • 2nd Axis: L-509.40DG10 ("Y")
  • 3rd axis: L-509.20DG10 ("Z")
  • 4th axis: M-060.DG ("Rotation")
  • 5th axis: M-406.4PD ("Focus")

The 5th axis ("Focus") is set up as part of the detection system. In case the detection system is not set up yet, you can test & and connect the M-406 stage without integrating it into the system.

Getting the PI stages to work

  • open PI Micro Move
  • connect a C-884 with the correct serial number
  • Assign axes:
    • 1st axis: L-509.20DG10 ("X")
    • 2nd Axis: L-509.40DG10 ("Y")
    • 3rd axis: L-509.20DG10 ("Z")
    • 4th axis: M-060.DG ("Rotation")
    • 5th axis: M-406.4PD ("Focus")
  • Save settings permanently on the controllername
  • Select all axes
  • Hit Automatic for automatic referencing
  • Switch on Servos and start referencing
  • Set velocities:
    • L-509.20DG10: 3 mm/s
    • L-509.40DG10: 3 mm/s
    • M-060.DG: 10 deg/s
    • M-406.4PD: 15 mm/s
  • move all linear stages to positions close to the center
    • L-509.20DG10: 26 mm
    • L-509.40DG10: 51 mm
    • M-406.4PD: 50 mm
  • close Micromove
  • ⚠️ For mesoSPIM-control to work, the same axis / stage assignments have to be set in the microscope configuration file!
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