-
Notifications
You must be signed in to change notification settings - Fork 4
mesoSPIM_excitation_path_L3_L4_distance
Fabian F. Voigt edited this page Mar 11, 2019
·
1 revision
The next step is to set the distance between L3 and L4 correctly.
For this, an alignment collimator and a shear plate can be used.
L3 and L4 form a 1:1 pupil relay between the tunable lens and galvo scanner. The flint (negative) elements should face each other.
Take M5 out again and put the alignment collimator up so that the beam enters L3 and gets reflected by M4 along the raised FLS-40 rail.
Then, put in the lens mount carrying L3 and attach / place a
shearing interferometer in the beam path.
Another way of adding a collimator to set the L4 to L3 distance:
-
Background
- mesoSPIM history
- Optical design
- Electronics
-
Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
-
Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants